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Manduca sexta Segmental Ganglia - Recording p. 1
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Below are photos showing how the ganglia are pinned in the recording dish.
How the nerve cord is pinned in the recording dish
Click HERE for a one minute video (10MB)

The microscope stage: in the middle you can see the dish in which the ganglia are pinned.   The 3 suction recording electrodes come in from the right. The intracellular recording electrode is located at the far left and not visible in this photo.

In the center of the photo is the dish containing the ganglia.  Some of the ganglia are isolated by a thick "wall" of petroleum jelly. In the dish are also a ground wire and a perfusion system that  circulates the bath solution in part of the dish. A close-up photo of the dish contents is shown on the recording 3 page. The ends of the three suction recording electrodes are marked with blue arrows.
The six ganglia are shown by the orange arrows. From the left, going counterclockwise, they are ganglia 2,3,4,5,6 and the fused ganglia #7-11.

Here the web designer has colored the wall of petroleum jelly orange.

Here the web designer has colored the two different,  solutions yellow and blue. The perfusion system refreshes the solution that is colored yellow.
Close-up view of all the ganglia
Click HERE for 15 second video (2.6MB)

A close-up view of ganglia 5 and 4 (from left to right).

A close up view of the entire nerve cord.
Clockwise from the left: the fused ganglia numbers 7-11, then ganglion 6, 5, 4, 3 and 2.


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