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Methods in Neuroscience
Squid Giant Synapse - DISSECTION - Page 3
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The final step in preparation for recording is to pin the ganglion to the center of the recording dish.
Pinning the desheathed ganglion
Click HERE for 40 sec video (4.5MB)

Both sets of axons were tied off, and are now pinned to the dish.

The ends of the cut post-synaptic axons are pinned out.

The final preparation.

A close up view of the ganglion.

Here some of the pre-synaptic axons have been colored orange.
 

The preparation in the recording dish. The arrow shows the ganglion. Presynaptic axon bundle is running approximately vertically from the ganglion; postsynaptic bundle is running approximately horizontally from the ganglion.
   

 

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