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Methods in Neuroscience Squid Giant Synapse - DISSECTION - Page 3 |
Dissection 1 Dissection 2 Dissection 3 Recording 1 Recording 2 Squid home |
The final step in preparation for recording is to pin the ganglion to the center of the recording dish. |
Pinning the desheathed ganglion Click HERE for 40 sec video (4.5MB) |
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Both sets of axons were tied off, and are now pinned to the dish. |
The ends of the cut post-synaptic axons are pinned out. |
The final preparation. |
A close up view of the ganglion. |
Here some of the pre-synaptic axons have been colored orange. |
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The preparation in the recording dish. The arrow shows the ganglion. Presynaptic axon bundle is running approximately vertically from the ganglion; postsynaptic bundle is running approximately horizontally from the ganglion. |
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