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Methods in Neuroscience
Goldfish Mauthner Cell - RECORDING page 3
Dissection      Recording 1       Recording 2      Recording 3     Goldfish home
Recording the Mauthner cell response to a sound stimulus
Click HERE for 15 second video (2.5MB)

The upper trace is the response recorded intracellularly in the Mauther cell lateral dendrite.  The lower trace is the same response recorded at a lower gain.  The VIIIth nerve afferents convey the hair cell response to the distal portion of the Mauthner cell lateral dendrite. This response is below threshold for spike initiation in the Mauthner cell.

In the absence of a sound stimulus, stimulus, there is no response in the Mauthner cell.

Similar data as recorded on the computer monitor.  Here the lower trace is the auditory stimulus, recorded with a microphone near the fish's head.


Intracellular recording in the Mauthner cell dendrite
Click HERE for Part 1- 40 second video (6.5MB)
Click HERE for Part 2 - 30 second video (5.4MB)
Click HERE for Part 3 - 20 second video (3MB)

In the beginning, no response is to a small stimulus given through the recording electrode as it goes toward the MC dendrite.

As the electrode goes through the tissue it can become clogged. This is seen as an increase in the decay constant of the stimulus artifact (arrow).

As the Mauthner cell is approached, spontaneous activity can be recorded extracellularly.

The spontaneous action potentials occur at various times after the stimulus pulse.

As the Mauthner cell is penetrated, a small depolarization can begin to be seen following the stimulus artifact.

The small depolarization grows in amplitude as the recording improves.

and grows...(now the oscilloscope displays both a high gain and a low gain trace).

and grows...

and grows (now only the low gain trace is shown)

The full AP recorded in the Mauthner cell dendrite. The calibration pulse is 12mV.

The AP amplitude is only 12 mV because it is being recorded in the dendrite.  The AP amplitude in the soma would be normal, i.e. 80 or 90 mV.

The resting potential of the Mauthner cell was a characteristic -71 mV, as shown here on the amplifier.


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